Recombinant Human GSTZ1 Protein Summary
| Description |
A recombinant protein with a His tag and corresponding to amino acids 1 – 216 of GSTZ1.
Source: E. coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MQAGKPILYS YFRSSCSWRV RIALALKGID YETVPINLIK DGGQQFSKDF QALNPMKQVP TLKIDGITIH QSLAIIEYLE ETRPTPRLLP QDPKKRASVR MISDLIAGGI QPLQNLSVLK QVGEEMQLTW AQNAITCGFN ALEQILQSTA GIYCVGDEVT MADLCLVPQV ANAERFKVDL TPYPTISSIN KRLLVLEAFQ VSHPCRQPDT PTELRA |
| Preparation Method |
E.coli
|
| Protein/Peptide Type |
Recombinant Protein
|
| Gene |
GSTZ1
|
| Purity |
>95% pure by SDS-PAGE
|
Applications/Dilutions
| Theoretical MW |
26.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles.
|
| Buffer |
Phosphate Buffered Saline pH7.4 containing 10% glycerol
|
| Preservative |
No Preservative
|
| Purity |
>95% pure by SDS-PAGE
|
Notes
The purity of this protein is > 95% by SDS-PAGE. Molecular weight is 26.2 kDa (236aa), confirmed by MALDI-TOF.
Alternate Names for Recombinant Human GSTZ1 Protein
- EC 2.5.1.18
- EC 5.2.1.2
- glutathione S-alkyltransferase
- glutathione S-aralkyltransferase
- Glutathione S-transferase zeta 1
- glutathione transferase zeta 1
- GSTZ1-1maleylacetoacetate isomerase
- MAAIglutathione S-aryltransferase
- MAI
- maleylacetone isomerase
- MGC2029
- S-(hydroxyalkyl)glutathione lyase
Background
GSTZ1, also known as maleylacetoacetate isomerase, is an enzyme that belongs to glutathione S-transferase (GSTs) super-family. This enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur). The reactions utilizing glutathione contribute the transformation of a wide variety of electrophiles, including reactive products of lipid, protein, carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress. It also plays a significant role in the catabolism of phenylalanine and tyrosine. Recombinant GSTZ1 protein was expressed in E.coli and purified by using conventional chromatography techniques.