Recombinant Human Cytokeratin 19 Protein Summary
| Description |
A denatured recombinant protein with a His tag corresponding to amino acids 1 – 400 of KRT19.
Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMTSYSYR QSSATSSFGG LGGGSVRFGP GVAFRAPSIH GGSGGRGVSV SSARFVSSSS SGAYGGGYGG VLTASDGLLA GNEKLTMQNL NDRLASYLDK VRALEAANGE LEVKIRDWYQ KQGPGPSRDY SHYYTTIQDL RDKILGATIE NSRIVLQIDN ARLAADDFRT KFETEQALRM SVEADINGLR RVLDELTLAR TDLEMQIEGL KEELAYLKKN HEEEISTLRG QVGGQVSVEV DSAPGTDLAK ILSDMRSQYE VMAEQNRKDA EAWFTSRTEE LNREVAGHTE QLQMSRSEVT DLRRTLQGLE IELQSQLSMK AALEDTLAET EARFGAQLAH IQALISGIEA QLGDVRADSE RQNQEYQRLM DIKSRLEQEI ATYRSLLEGQ EDHYNNLSAS KVL |
| Protein/Peptide Type |
Recombinant Protein
|
| Gene |
KRT19
|
| Purity |
>90%, by SDS-PAGE
|
Applications/Dilutions
| Theoretical MW |
46.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
|
| Buffer |
20mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol
|
| Preservative |
No Preservative
|
| Concentration |
0.5 mg/ml
|
| Purity |
>90%, by SDS-PAGE
|
Alternate Names for Recombinant Human Cytokeratin 19 Protein
- CK19
- CK-19
- Cytokeratin 19
- Cytokeratin-19,40-kDa keratin intermediate filament
- EndoC
- K19
- K19cytokeratin-19
- K1CS
- keratin 19
- keratin, type I cytoskeletal 19
- keratin, type I, 40-kd
- keratin-19
- Krt19
- MGC15366
Background
KRT19 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Recombinant human KRT19 protein, fused to His-tag at N-terminus, was expressed in E.coli.