Product: LY2835219 (free base)
Recombinant CXCL2/GRO beta/MIP-2/CINC-3 Protein Summary
| Description |
A biologically active protein to CXCL2.
Source: E. coli.Amino Acid Sequence: LGASWHRPDK CCLGYQKRPL PQVLLSSWYP TSQLCSKPGV IFLTKRGRQV CADKSKDWVK KLMQQLPVTA |
| Preparation Method |
Novus biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance.
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| Details of Functionality |
CXCL2 Protein is fully biologically active when compared to standard. The specific activity is determined by the inhibitory effect on monocyte migration response to human MIP-1 alpha using a concentration range of 1.0ug-10.0ug/ml of viral MIP-2 will inhibit 25ng/ml of human MIP-1 alpha.
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| Protein/Peptide Type |
Biologically Active Protein
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| Gene |
CXCL2
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| Purity |
>95% pure by SDS-PAGE
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| Endotoxin Note |
Less than 1 EU/ug of endotoxin as determined by LAL method.
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Applications/Dilutions
| Theoretical MW |
8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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| Concentration |
LYOPH
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| Purity |
>95% pure by SDS-PAGE
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| Reconstitution Instructions |
Reconstitute with sterilized distilled water or 0.1% BSA aqueous buffer to a final concentration of 0.1 – 1.0 mg/ml.
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Notes
This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
Alternate Names for Recombinant CXCL2/GRO beta/MIP-2/CINC-3 Protein
- chemokine (C-X-C motif) ligand 2
- CINC-2a
- CINC3
- CINC-3
- C-X-C Motif Chemokine 2
- CXCL2
- GRO beta
- GRO2 oncogene
- GRO2
- GROB
- Gro-beta
- Growth-regulated protein beta
- Macrophage Inflammatory Protein 2
- Macrophage inflammatory protein 2-alpha
- Macrophage Inflammatory Protein
- melanoma growth stimulatory activity beta
- MGSA beta
- MGSA-b
- MGSA-beta
- MIP2
- MIP-2
- MIP2A
- MIP-2a
- MIP2-alpha
- SCYB2
Background
Viral CXCL2 (MIP-2) is one of the three chemokine-like proteins expressed by the human herpesvirus 8 (Kaposis sarcoma-associated herpesvirus, KSHV) and the other is ViMIP-1 and ViMIP-3. It shares 41 % and 48 % with human MIP-1alpha and ViMIP-1, respectively. Viral CXCL2 (MIP-2) has been shown to have antagonist activity towards a wide range of chemokine receptors and has functions of blocking infection by several different human immunodeficiency virus type 1 (HIV-1) strains. It may form part of the response to host defenses contributing to virus-induced neoplasia and may have relevance to KSHV and HIV-I interactions. Additionally, Viral CXCL2 (MIP-2) has been shown to activate and chemoattract human eosinphils.