Rat CD3 T Cell Isolation Kit Summary
| Description |
The MagCellect Rat CD3+ T Cell Isolation Kit is designed to isolate CD3+ T cells via a negative selection principle. The resulting cell preparation is highly enriched with CD3+ T cells. Typical recovery of the targeted cell population ranges from 40-61% and the purity of recovered CD3+ T cells ranges from 80-90%.
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| Kit Type |
Isolation Kit
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| Gene |
CD3
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Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze.
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Kit Components
| Components |
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Notes
Other Supplies Required: MagCellect Magnet, Mouse Erythrocyte Lysing Kit, 12 x 75 mm (5 ml) or 17 x 100 mm (15 ml) polystyrene round bottom tubes, Sterile Pasteur pipettes or transfer pipettes, 15 ml conical centrifuge tubes, Sterile deionized or distilled water, Hanks BSS or equivalent, Bovine serum
Background
The T cell receptor (TCR)/CD3 complex consists of two variable antigen-recognition receptor chains (TCR-alpha/TCR-beta or TCR-gamma/TCR-delta) that are non-covalently linked to at least four different invariant chains: CD3 gamma, CD3 delta, CD3 epsilon, and the CD3 zeta chain that exists as either a homodimer or as a heterodimer with its splice-variant the CD3 eta chain. The CD3 complex functions to transduce intracellular signals during TCR antigen recognition. CD3 is expressed early in thymocyte development, and on a subset of NK cells. CD3 epsilon is one of at least three invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction. MagCellect products are designed for the isolation of cells in a “liquid phase”. MagCellect technology is based on the use of ferrofluids or magnetic nanoparticles that have no magnetic memory (superparamagnetic) and behave like colloidal particles. This feature allows the ferrofluids to remain in solution without the need for mixing and additionally allows for efficient diffusion kinetics during the binding reaction. The proprietary manufacturing technology of MagCellect Ferrofluids generates particles with higher ligand binding capacity per mass compared to many other larger diameter magnetic particles.