Rabbit anti-Human IgA Secondary Antibody [Biotin]

Product: LUF6000

Rabbit anti-Human IgA Secondary Antibody [Biotin] Summary

Immunogen
Human IgA alpha heavy chain
Specificity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Human IgA and Human Serum. No reaction was observed against Human IgG or IgM. Specificity was confirmed at less than 1% against other human heavy or light chain isotypes by ELISA.
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:2000 – 1:10000
  • ELISA 1:20000 – 1:100000
  • Immunohistochemistry 1:1000 – 1:5000
Application Notes
This product has been assayed against 1.0 ug of Human IgA in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS ELISA PEROXIDASE SUBSTRATE for 30 minutes at room temperature. A working dilution of 1:10,000 – 1:100,000 of the reconstitution concentration is suggested for this product.
Readout System
  • Streptavidin Full length Protein
  • Streptavidin Full length Protein
  • Streptavidin Full length Protein

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2. 10 mg/mL Bovine Serum Albumin (BSA) – Immunoglobulin and Protease free
Preservative
0.01% Thimerosal
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 1.0 ml deionized water.

Notes

Label Molecular Weight: 455, Restore with 1.0 mL of deionized water (or equivalent).

Alternate Names for Rabbit anti-Human IgA Secondary Antibody [Biotin]

  • Arc-1
  • cadherin 1, E-cadherin (epithelial)
  • cadherin 1, type 1, E-cadherin (epithelial)
  • cadherin-1
  • calcium-dependent adhesion protein, epithelial
  • CAM 120/80
  • CD324 antigen
  • CD324
  • CDHE
  • cell-CAM 120/80
  • ECAD
  • E-cadherin
  • Epithelial cadherin
  • IgA
  • LCAM
  • UVOE-Cadherin
  • uvomorulin

PMID: 21159605