Rabbit anti-Bovine IgG (H+L) Secondary Antibody [HRP]

Product: CPI-637

Rabbit anti-Bovine IgG (H L) Secondary Antibody [HRP] Summary

Immunogen
Bovine IgG-heavy and light chain
Specificity
By immunoelectrophoresis and ELISA this reacts specifically with bovine IgG and with light chains common to other bovine immunoglobulins. No was detected against non-immunoglobulin serum proteins. This may cross react with IgG from other species.
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:5000 – 1:50000
  • ELISA 1:10000- 1:100000
  • Immunocytochemistry/Immunofluorescence 1:200 – 1:5000
  • Immunohistochemistry 1:200 – 1:5000
Application Notes
WB: Colorimetric – use at a dlution of 1:1,000 – 1:10,000. Chemiluminescent – use at a dilution of 1:1,000 – 1:30,000.
Readout System
  • NovaLume Plus Chemiluminescent Reagent

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Phosphate Buffered Saline (PBS)
Preservative
0.1% Pro-Clean 400
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

PMID: 24330646

Rabbit anti-Bovine IgG (H+L) Secondary Antibody [HRP]

Product: Epinastine

Rabbit anti-Bovine IgG (H L) Secondary Antibody [HRP] Summary

Immunogen
Purified Bovine IgG (H&L)
Specificity
Based on Immunoelectrophoresis, this antibody reacts with heavy chains on bovine IgG and light chains on all bovine immunoglobulins.
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:200-1:5000
  • ELISA 1:200-1:5000
  • Immunohistochemistry 1:500-1:5000
Application Notes
This conjugate is suitable for all immunoassay applications.

Packaging, Storage & Formulations

Storage
Store at 4C. After reconstitution, dilute with 50% glycerol and store at -20C. Avoid repeated freeze-thaw cycles.
Buffer
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free.
Preservative
0.1% Kathon
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 1.1 ml deionized water and let stand 30 minutes at RT to disolve. Centrifuge to remove any particulates.

Notes

Purity > 95% based on SDS-PAGE. Rabbit serum was obtained from healthy animals of US origin and under the care of a registered veterinarian. After reconstituted, prepare fresh working dilution daily.

PMID: 3011168