R-Spondin 4 Antibody

Product: TG100-116

R-Spondin 4 Antibody Summary

Immunogen
E. coli-derived recombinant mouse R-Spondin 4
Tyr21-Pro197
Accession # Q8BJ73
Specificity
Detects mouse R-Spondin 4 in direct ELISAs.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
RSPO4
Purity
Immunogen affinity purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
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Applications/Dilutions

Dilutions
  • Neutralization 5.0-20 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for R-Spondin 4 Antibody

  • C20orf182
  • chromosome 20 open reading frame 182
  • Cristin 4
  • CRISTIN4
  • dJ824F16.3
  • FLJ16018
  • hRspo4
  • Roof plate-specific spondin-4
  • RSPO4
  • RSpondin 4
  • R-Spondin 4
  • R-spondin family, member 4
  • R-spondin-4

Background

R-Spondin 4 (RSPO4, roof plate-specific spondin 4), also called cysteine-rich and single thrombospondin domain containing-4 (Cristin 4), is an ~33 kDa secreted heparin-binding protein that shares ~35% amino acid (aa) identity with three other R-Spondin family members (1-3). All are positive modulators of Wnt/ beta -catenin signaling, but R-Spondin 4 may be somewhat weaker than other R-Spondins (2). R‑Spondins regulate Wnt/ beta -catenin by competing with the Wnt antagonist DKK-1 for binding to the Wnt co-receptors LRP-6 and Kremen, reducing their DKK‑1‑mediated internalization (1, 4). Like other R‑Spondins, mouse R-Spondin 4 (234 aa) contains a signal sequence (aa 1-19), two adjacent cysteine-rich furin-like domains (aa 85-128) with one potential tyrosine phosphorylation site (aa 114), followed by a thrombospondin (TSP-1) motif (aa 137‑197) and a region rich in basic residues (aa 199‑234). The furin-like domains are sufficient for beta -catenin stabilization (2). Mature mouse R‑Spondin 4 shares 81%, 97%, 79%, 77% and 76% aa identity with human, rat, bovine, equine and canine R-Spondin 4, respectively. There is one potential isoform where Arg substitutes for the C‑terminal 82 amino acids (5). Each R‑Spondin has a distinct expression pattern (6). In the mouse, R‑Spondin 4 mRNA is found during development of limb bud mesenchyme, nail beds, heart and teeth (6‑8). In humans, mutations of R‑Spondin 4 have been found to cause anonychia, a condition in which fingernails and toenails are absent (8‑10).

PMID: 16574785