Product: Impurity C of Alfacalcidol
RNF7 Antibody Summary
| Immunogen |
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 102-113 of Human ROC2 (C-terminal) coupled to KLH.
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| Specificity |
This product reacts with human, mouse, C.elgans and zebra fish ROC2. Cross reactivity may also occur with ROC2 from other sources.
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
RNF7
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| Purity |
Sterile filtered
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Applications/Dilutions
| Dilutions |
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| Application Notes |
The antibody immunoprecipitates in vitro translated protein and protein from overexpressing cell lysates. Coimmunoprecipitation of related proteins does occur. A 12.6 kDa band corresponding – human ROC2 is detected. Most cell lines expressing ROC2 can be used as a positive control. Researchers should determine optimal titers for other applications.
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| Publications |
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Reactivity Notes
Reactive in human
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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| Buffer |
No buffer
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| Preservative |
0.01% Sodium Azide
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| Concentration |
85 mg/ml
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| Purity |
Sterile filtered
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Alternate Names for RNF7 Antibody
- CKBBP1zinc RING finger protein SAG
- CKII beta-binding protein 1
- RBX2
- Regulator of cullins 2
- ring finger protein 7rbx2
- ROC2RING-box protein 2
- SAGsensitive to apoptosis, zinc RING finger protein SAG, regulator of cullins 2
- Sensitive to apoptosis gene protein
Background
The protein encoded by this gene is a highly conserved ring finger protein. It is an essential subunit of SKP1-cullin/CDC53-F box protein ubiquitin ligases, which are a part of the protein degradation machinery important for cell cycle progression and signal transduction. This protein interacts with, and is a substrate of, casein kinase II (CSNK2A1/CKII). The phosphorylation of this protein by CSNK2A1 has been shown to promote the degradation of IkappaBalpha (CHUK/IKK-alpha/IKBKA) and p27Kip1(CDKN1B). Alternatively spliced transcript variants encoding distinct isoforms have been reported.