RIPK1/RIP1 Antibody Summary
| Immunogen |
Synthetic peptide surrounding amino acid 645 of human RIP.
|
| Clonality |
Polyclonal
|
| Host |
Rabbit
|
| Gene |
RIPK1
|
| Purity |
Immunogen affinity purified
|
| Innovators Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovators Reward
|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Western blot analysis (2-6 ug/ml), immunoprecipitation and Immunohistochemistry. However, the optimal conditions should be determined individually.
|
Packaging, Storage & Formulations
| Storage |
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
|
| Buffer |
PBS (pH 7.2), 0.5% BSA and 30% Glycerol
|
| Preservative |
0.01% Thimerosal
|
| Concentration |
0.5 mg/ml
|
| Purity |
Immunogen affinity purified
|
Alternate Names for RIPK1/RIP1 Antibody
- Cell death protein RIP
- EC 2.7.11.1
- receptor (TNFRSF)-interacting serine-threonine kinase 1
- receptor interacting protein
- Receptor-interacting protein 1
- receptor-interacting serine/threonine-protein kinase 1
- RIP
- Rip1 kinase
- RIP1
- RIP-1
- RIPFLJ39204
- RIPK1
- Serine/threonine-protein kinase RIP
Background
RIP (Receptor Interacting Protein) is a 74 kD Ser/Thr kinase which interacts with CD95 (Fas/APO1) receptor and the tumor necrosis factor receptor (TNFR1). It is a cell death domain adapter protein which can bind to the adapter proteins TRADD, RAID (CRADD) and TRAF2. RIP contains an N terminal region with homology to protein kinases, an intermediate domain capable of association with MAPKKK and a C terminal region containing an intracellular death domain motif. RIP activates both p38 MAP Kinase and SAPK families. In vitro, RIP induces apoptosis, as well as SAPK/JNK and NF-kB activation. NF-kB activation through TRADD, TRAF2 and RIP can be triggered also by DR3/APO3 upon activation with APO3/Tweak ligand. DR4 and DR5 also use FADD, TRADD, and RIP in their signal transduction pathways. RIP deficient mice fail to thrive, displaying extensive apoptosis in both lymphoid and adipose tissues and dying at 1-3 days of age. RIP possesses kinase activity as it autophosphorylates itself on serine and threonine residues.