RFP Antibody

Product: GSK 525768A

RFP Antibody Summary

Immunogen
The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
Specificity
This product has been assayed against 1.0 ug of Biotinylated BSA in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and (ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads followed by pepsin digestion and chromatographic separation.
Clonality
Polyclonal
Host
Chicken
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:3000
  • ELISA 1:10000
Application Notes
Polyclonal anti-RFP is designed – detect RFP and its variants. This antibody can be used – detect RFP by ELISA for the direct binding of antigen. Biotin conjugated polyclonal anti-RFP used in a sandwich ELISA with unconjugated anti-RFP is well suited – titrate RFP in solution. The detection antibody conjugated – biotin is subsequently reacted with streptavidin conjugated HRP. Fluorochrome conjugated polyclonal anti-RFP can be used – detect RFP by Immunofluorescence microscopy in cell expression systems and can detect RFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-RFP relative – the fluorescence of RFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP – detect RFP or RFP containing proteins on western blots. This product shows optimal performance by western blot.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for RFP Antibody

  • dsRedGene Symbol
  • Recombinant RFP Protein
  • Red Fluoroscent Protein

PMID: 26150770