RFP Antibody Summary
| Immunogen |
The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
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| Specificity |
This product has been assayed against 1.0 ug of Biotinylated BSA in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and (ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads followed by pepsin digestion and chromatographic separation. |
| Clonality |
Polyclonal
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| Host |
Chicken
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| Purity |
Immunogen affinity purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
Polyclonal anti-RFP is designed – detect RFP and its variants. This antibody can be used – detect RFP by ELISA for the direct binding of antigen. Biotin conjugated polyclonal anti-RFP used in a sandwich ELISA with unconjugated anti-RFP is well suited – titrate RFP in solution. The detection antibody conjugated – biotin is subsequently reacted with streptavidin conjugated HRP. Fluorochrome conjugated polyclonal anti-RFP can be used – detect RFP by Immunofluorescence microscopy in cell expression systems and can detect RFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-RFP relative – the fluorescence of RFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP – detect RFP or RFP containing proteins on western blots. This product shows optimal performance by western blot.
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Packaging, Storage & Formulations
| Storage |
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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| Buffer |
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
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| Preservative |
0.01% Sodium Azide
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| Purity |
Immunogen affinity purified
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Alternate Names for RFP Antibody
- dsRedGene Symbol
- Recombinant RFP Protein
- Red Fluoroscent Protein