Protein O-Fucosyltransferase 1/POFUT1 Antibody

Product: Vinpocetine

Protein O-Fucosyltransferase 1/POFUT1 Antibody Summary

Immunogen
POFUT1 (NP_758436.1, 1 a.a. ~ 194 a.a) full-length human protein.MGAAAWARPLSVSFLLLLLPLPGMPAGSWDPAGYLLYCPCMGRFGNQADHFLGSLAFAKLLNRTLAVPPWIEYQHHKPPFTNLHVSYQKYFKLEPLQAYHRVISLEDFMEKLAPTHWPPEKRVAYCFEVAAQRSPDKKTCPMKEGNPFGPFWDQFHVSFNKSELFTGISFSASYREQWSQRRENHSCVTLLFPR
Specificity
POFUT1 – protein O-fucosyltransferase 1,
Clonality
Polyclonal
Host
Rabbit
Gene
POFUT1
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA
Application Notes
Antibody reactive against recombinant protein with GST tag on ELISA and western blot and also on transfected lysate in western blot. GST tag alone is used as a negative control.
Publications
Read Publication using H00023509-D01P.

Reactivity Notes

Human, Mouse.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Protein O-Fucosyltransferase 1/POFUT1 Antibody

  • FucT-1
  • FUT12
  • FUT12EC 2.4.1.221
  • GDP-fucose protein O-fucosyltransferase 1
  • KIAA0180MGC2482
  • o-fucosyltransferase protein
  • O-Fuc-T
  • O-FucT-1
  • O-FUT
  • Peptide-O-fucosyltransferase 1
  • POFUT1
  • Protein OFucosyltransferase 1
  • Protein O-Fucosyltransferase 1

Background

This gene encodes a member of the glycosyltransferase O-Fuc family. This enzyme adds O-fucose through an O-glycosidic linkage to conserved serine or threonine residues in the epidermal growth factor-like repeats of a number of cell surface and secreted proteins. O-fucose glycans are involved in ligand-induced receptor signaling. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq]

PMID: 18930747

Protein O-Fucosyltransferase 1/POFUT1 Antibody

Product: Proguanil D8

Protein O-Fucosyltransferase 1/POFUT1 Antibody Summary

Immunogen
Antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human POFUT1. The immunogen is located within amino acids 90 – 140 of POFUT1.
Specificity
At least two isoforms of POFUT1 are known to exist. This antibody is predicted to not cross-react with POFUT2.
Clonality
Polyclonal
Host
Rabbit
Gene
POFUT1
Purity
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

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Applications/Dilutions

Dilutions
  • Western Blot 0.5-1 ug/ml
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 2.5 ug/ml
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
Protein O-Fucosyltransferase 1/POFUT1 Peptide (NBP1-76940PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Protein O-Fucosyltransferase 1/POFUT1 Antibody

  • FucT-1
  • FUT12
  • FUT12EC 2.4.1.221
  • GDP-fucose protein O-fucosyltransferase 1
  • KIAA0180MGC2482
  • o-fucosyltransferase protein
  • O-Fuc-T
  • O-FucT-1
  • O-FUT
  • Peptide-O-fucosyltransferase 1
  • POFUT1
  • Protein OFucosyltransferase 1
  • Protein O-Fucosyltransferase 1

Background

POFUT1, an endoplasmic reticulum-residing member of the glycosyltransferase O-Fuc family, adds O-fucose through an O-glycosidic linkage to conserved serine or threonines in epidermal growth factor-like repeats of several cell surface and secreted proteins. Unlike its homolog POFUT2, POFUT1 can also catalyze the transfer of fucose to thrombospondin type 1 repeats. Many of the substrates of POFUT1 are involved in ligand-induced receptor signaling. One such protein is Notch; mouse ES cells lacking POFUT have normal levels of Notch receptors at the cell surface, but these receptors do not bind Notch ligands or exhibit Notch signaling. At least two isoforms of POFUT1 are known to exist. This antibody is predicted to not cross-react with POFUT2.

PMID: 20881099