Pdi1p Antibody (38H8)

Product: Reparixin (L-lysine salt)

Pdi1p Antibody (38H8) Summary

Immunogen
Yeast membrane preparations
Localization
endoplasmic reticulum and nuclear envelope
Marker
Endoplasmic Reticulum Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Tissue culture supernatant
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Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:500-1:1000
Application Notes
This Pdi1p (38H8) antibody is useful in Western blot and Immunocytochemistry/Immunofluorescence. In WB of yeast protein extracts a ~65 kDa band can be seen. In ICC/IF using yeast cells, endoplasmic reticulum staining can be seen.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Theoretical MW
65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Yeast.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
10mM Sodium Azide
Purity
Tissue culture supernatant

Alternate Names for Pdi1p Antibody (38H8)

  • MFP 1
  • MFP1
  • PDI 1
  • PDI
  • PDI1
  • Protein disulfide isomerase
  • Thioredoxin related glycoprotein 1
  • TRG 1
  • TRG1

Background

One of the important aspects of secretory proteins folding process is the formation of native disulfide bonds which is catalyzed by enzyme protein disulfide isomerase (PDI) and yeast Saccharomyces cerevisiae has about 5 protein disulfide isomerase (PDI) family members (EUG1, MPD1, MPD2, EPS1 and PDI1/PDI1p). Pdi1p is a typical member of its family which is an essential gene and is considered homologus to human PDI with no significant differences; however, the two proteins are only 29% identical. It is a major ER protein with chaperon/co-chaperon activity and is involved in the retention of resident ER proteins as well as in recognizing proteins targeted for ER-associated degradation (ERAD). Yeast Pdi1p is a folding catalyst which introduces disulfide bonds into its substrates and rearranges or reduces pre-existing ones, and besides its oxidoreductase activity, Pdi1p acts as a molecular chaperone that also facilitates folding of proteins which do not contain disulfide bonds.

PMID: 9864280