Patched 1/PTCH Antibody

Product: Pralatrexate

Patched 1/PTCH Antibody Summary

Immunogen
Peptide with sequence ELQDVECEERPR corresponding to C-Terminus according to NP_000255.1, NP_001077072.1, NP_001077071.1, NP_001077073.1, NP_001077074.1, NP_001077075.1, NP_001077076.1.
Specificity
This antibody is expected to recognise all reported isoforms. Variants (NP_001077073.1; NP_001077074.1; NP_001077075.1; NP_001077076.1) encode the same isoform.
Clonality
Polyclonal
Host
Goat
Gene
PTCH1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100 – 1:2000
  • Immunohistochemistry 3 – 6 ug/ml
  • Immunohistochemistry-Paraffin 3 – 6 ug/ml
  • Peptide ELISA Detection limit 1:8000
Application Notes
IHC-P: Human colon shows textured nuclear staining of the absorptive cells. IHC successful usage on human glioma sections is reported in scientific literature (PMID: 24867209).
Positive Control
Patched 1/PTCH Lysate (NBL1-14924)
Publications
Read Publication using NB100-2451.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at [email protected] for more details.

Alternate Names for Patched 1/PTCH Antibody

  • BCNS
  • BCNSFLJ42602
  • FLJ26746
  • HPE7
  • mes
  • NBCCS
  • patched (Drosophila) homolog
  • Patched 1
  • patched homolog (Drosophila)
  • patched homolog 1 (Drosophila)
  • patched homolog 1
  • protein patched homolog 1
  • PTC
  • ptc1
  • PTCH protein +12b
  • PTCH protein +4
  • PTCH protein -10
  • PTCH
  • PTCH1
  • PTCH11

PMID: 8643619