PSMF1 Isoform 1 Antibody

Product: Tacalcitol

PSMF1 Isoform 1 Antibody Summary

Immunogen
Peptide with sequence C-DHLPPPGYDDMYL corresponding to C-Terminus according to NP_006805.2.
Specificity
This antibody is expected to recognize isoform 1 (NP_006805.2 and NP_848693.2).
Clonality
Polyclonal
Host
Goat
Gene
PSMF1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1 – 3 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 35 kDa band observed in human kidney lysates (calculated MW of 29.8 kDa band according to NP_006805.2). In transfected HEK293 transiently expressing PSMF1 a band of approx. 35 kDa is observed. This band is not observed in the non-transfected HEK293.
Positive Control
Kidney Lysate (NB820-59231)
PSMF1 Lysate (NBL1-14910)
Publications
Read Publications using NB100-1178.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PSMF1 Isoform 1 Antibody

  • PI31
  • proteasome (prosome, macropain) inhibitor subunit 1 (PI31)
  • proteasome (prosome, macropain) inhibitor subunit 1
  • PSMF1

Background

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a protein that inhibits the activation of the proteasome by the 11S and 19S regulators. Alternative transcript variants have been identified for this gene.

PMID: 20886088