PSMF1 Isoform 1 Antibody Summary
| Immunogen |
Peptide with sequence C-DHLPPPGYDDMYL corresponding to C-Terminus according to NP_006805.2.
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| Specificity |
This antibody is expected to recognize isoform 1 (NP_006805.2 and NP_848693.2).
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| Clonality |
Polyclonal
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| Host |
Goat
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| Gene |
PSMF1
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| Purity |
Immunogen affinity purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
WB: Approx. 35 kDa band observed in human kidney lysates (calculated MW of 29.8 kDa band according to NP_006805.2). In transfected HEK293 transiently expressing PSMF1 a band of approx. 35 kDa is observed. This band is not observed in the non-transfected HEK293.
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| Positive Control |
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| Publications |
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Reactivity Notes
Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Bovine.
Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles.
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| Buffer |
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
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| Preservative |
0.02% Sodium Azide
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| Concentration |
0.5 mg/ml
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| Purity |
Immunogen affinity purified
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Alternate Names for PSMF1 Isoform 1 Antibody
- PI31
- proteasome (prosome, macropain) inhibitor subunit 1 (PI31)
- proteasome (prosome, macropain) inhibitor subunit 1
- PSMF1
Background
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a protein that inhibits the activation of the proteasome by the 11S and 19S regulators. Alternative transcript variants have been identified for this gene.