PMEL17/SILV Antibody (SPM286) – Azide and BSA Free Summary
| Immunogen |
Membranes from a human melanoma metastasis
|
| Localization |
Cytoplasmic
|
| Marker |
Melanoma Marker
|
| Specificity |
By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.This Mab labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
|
| Isotype |
IgG2b Kappa
|
| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Gene |
PMEL
|
| Purity |
Protein A purified
|
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|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Immunohistochemistry (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
|
| Buffer |
10mM PBS – BSA free
|
| Preservative |
No Preservative
|
| Concentration |
1.0 mg/ml
|
| Purity |
Protein A purified
|
Alternate Names for PMEL17/SILV Antibody (SPM286) – Azide and BSA Free
- D12S53EP1
- gp100
- ME20
- ME20-M
- melanocyte protein mel 17
- Melanocyte protein Pmel 17
- Melanocytes lineage-specific antigen GP100
- Melanoma-associated ME20 antigen
- melanosomal matrix protein17
- PMEL17P100
- premelanosome proteinME20M
- SI
- SIL
- silver (mouse homolog) like
- silver homolog (mouse)
- Silver locus protein homolog
- silver, mouse, homolog of
- SILVPmel17