PMCA1 Antibody Summary
| Immunogen |
Synthetic peptide corresponding to the residues A(5) N N S V A Y S G V K N S I K E A N(22) of rat PMCA1 ATPase.
|
| Localization |
Cell Membrane; Multi-pass membrane protein
|
| Specificity |
Detects PMCA 1a and 1b ATPase.
|
| Isotype |
IgG
|
| Clonality |
Polyclonal
|
| Host |
Rabbit
|
| Gene |
ATP2B1
|
| Purity |
Immunogen affinity purified
|
| Innovators Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovators Reward
|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
WB: Detects an approx. 134 kDa and 130 kDa proteins representing PMCA 1a and 1b ATPase from rat samples.
|
Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles.
|
| Buffer |
PBS
|
| Preservative |
0.05% Sodium Azide
|
| Concentration |
1 mg/ml
|
| Purity |
Immunogen affinity purified
|
Alternate Names for PMCA1 Antibody
- ATPase, Ca++ transporting, plasma membrane 1
- EC 3.6.3
- EC 3.6.3.8
- Plasma membrane calcium ATPase isoform 1
- plasma membrane calcium ATPase
- Plasma membrane calcium pump isoform 1
- plasma membrane calcium pump
- plasma membrane calcium-ATPase
- PMCA1kb
- PMCA1plasma membrane calcium-transporting ATPase 1
Background
The calcium pump of the plasma membrane, termed PMCA ATPase, pumps calcium from the cytosol to the extracellular space. This membrane-bound enzyme is related to a number of other ATPases including the SERCA ATPase and the sodium/potassium pump.There are four different genes encoding PMCA ATPase and studies have revealed 20 isoforms of the pump generated by alternate splicing of the primary gene products. mRNA distribution studies show that gene products 1 and 4 are transcribed in most tissues, however, products 2 and 3 are more tissue specific. Transcription of the splicing variants has also been found to be tissue specific. In the pancreas, where insulin secretion is calcium dependent, the beta cells only express the 4b isoform, however the alpha and gamma cells express both 4a and 4b isoforms. Studies have also shown that different splice variants have different affinities for calcium and calmodulin.