PLGF Antibody (37203.111)

Product: Glycitin

PLGF Antibody (37203.111) Summary

Immunogen
Purified recombinant human placenta growth factor expressed in E. coli.
Localization
The three isoforms are secreted but PlGF2 appears to remain cell attached unless released by heparin.
Specificity
In immunoblotting, the shows no cross-reactivity with recombinanthuman VEGF, PDGFAA, PDGFAB, and PDGFBB.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PGF
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • ELISA 2 ug/ml
Application Notes
ELISA: For capture ELISAs, this product can be used as the capture antibody. Use at a working concentration in combination with a biotinylated PlGF polyclonal detection antibody at 100 ng/ml (100 ul/well). WB: The detection limit for recombinant human placental growth factor is approximately 20 ng/lane and 300 ng/lane under non-reducing and reducing conditions, respectively. Homodimeric recombinant PlGF migrates as an approximately 29 kDa protein.
Reviewed Applications
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using
NB120-10966 in the following applications:

  • ELISA

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2 um filtered solution in PBS and carbohydrates
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for PLGF Antibody (37203.111)

  • D12S1900
  • PGF
  • PGFL
  • placenta growth factor
  • placental growth factor
  • placental growth factor, vascular endothelial growth factor-related protein
  • PlGF
  • PlGF-2
  • PLGFplacental growth factor-like
  • SHGC-10760

PMID: 22374891