PKLR Antibody

Product: Diphenidol (hydrochloride)

PKLR Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence:GSPLSYRPVAIALDTKGPEIRTGILQGGPESEVELVKGSQVLVTVDPAFRTRGNANTVWVDYPNIVRVVPVGGRIYIDDGLISLVVQKIGPEGLVTQVENGGVLGSRKGVNLPGA
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PKLR
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100 – 1:250
  • Immunocytochemistry/Immunofluorescence 1-4 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
PKLR Recombinant Protein Antigen (NBP2-58892PEP)

Reactivity Notes

Mouse 86%, Rat 87%

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, containing 40% glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for PKLR Antibody

  • EC 2.7.1.40
  • PK1
  • PK1PKRL
  • PKL
  • PKLR
  • PKR
  • Pyruvate Kinase 1
  • pyruvate kinase isozyme R/L
  • pyruvate kinase isozymes R/L
  • pyruvate kinase type L
  • pyruvate kinase, liver and blood cell
  • pyruvate kinase, liver and RBC
  • Red cell/liver pyruvate kinase
  • RPK
  • R-type/L-type pyruvate kinase

PMID: 24481568

PKLR Antibody

Product: BCX 1470 (methanesulfonate)

PKLR Antibody Summary

Immunogen
Pyruvate Kinase from rabbit muscle
Localization
Cytoplasmic
Specificity
Cross reacts with all forms of muscle pyruvate kinase (pan M-PK). Cross reactivity against Pyruvate Kinase from other tissues and species may occur but have not been specifically determined.
Clonality
Polyclonal
Host
Goat
Gene
PKLR
Purity
IgG purified
Innovators Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

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Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:20000
  • ELISA 1:50000-1:200000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Against 1.0 ug of Pyruvate Kinase from rabbit muscle in standard sandwich. Optimal titers for other applications should be determined by the researcher.
Publications
Read Publication using
NB600-1447 in the following applications:

  • WB
    1 publication

Reactivity Notes

Cross-reacts with Rabbit. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
IgG purified

Alternate Names for PKLR Antibody

  • EC 2.7.1.40
  • PK1
  • PK1PKRL
  • PKL
  • PKLR
  • PKR
  • Pyruvate Kinase 1
  • pyruvate kinase isozyme R/L
  • pyruvate kinase isozymes R/L
  • pyruvate kinase type L
  • pyruvate kinase, liver and blood cell
  • pyruvate kinase, liver and RBC
  • Red cell/liver pyruvate kinase
  • RPK
  • R-type/L-type pyruvate kinase

PMID: 12466229

PKLR Antibody

Product: Triptophenolide

PKLR Antibody Summary

Immunogen
Synthetic peptides corresponding to PKLR(pyruvate kinase, liver and RBC) The peptide sequence was selected from the N terminal of PKLR (NP_870986).Peptide sequence STSIIATIGPASRSVERLKEMIKAGMNIARLNFSHGSHEYHAESIANVRE.
Clonality
Polyclonal
Host
Rabbit
Gene
PKLR
Purity
Protein A purified
Innovators Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Learn about the Innovators Reward

Applications/Dilutions

Dilutions
  • Western Blot 5 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 4-8 ug/ml
Application Notes
This is a rabbit polyclonal antibody against PKLR and was validated on Western Blot and immunohistochemistry-P

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Theoretical MW
58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP1-56319.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 2% Sucrose
Preservative
0.09% Sodium Azide
Purity
Protein A purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for PKLR Antibody

  • EC 2.7.1.40
  • PK1
  • PK1PKRL
  • PKL
  • PKLR
  • PKR
  • Pyruvate Kinase 1
  • pyruvate kinase isozyme R/L
  • pyruvate kinase isozymes R/L
  • pyruvate kinase type L
  • pyruvate kinase, liver and blood cell
  • pyruvate kinase, liver and RBC
  • Red cell/liver pyruvate kinase
  • RPK
  • R-type/L-type pyruvate kinase

Background

PKLR is a pyruvate kinase that catalyzes the production of phohsphoenolpyruvate from pyruvate and ATP. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA).The protein encoded by this gene is a pyruvate kinase that catalyzes the production of phohsphoenolpyruvate from pyruvate and ATP. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Alternatively spliced transcript variants encoding distinct isoforms have been described.

PMID: 22558207