PICK1 Antibody Summary
| Immunogen |
Synthetic peptide corresponding to residues M(1) F A D L D Y D I E E D K L G I P T(18) of mouse PICK1.
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| Localization |
Cytoplasmic; concentrated in the perinuclear region. Also present at excitatory synapses.
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| Specificity |
Detects protein interacting with Protein Kinase C (PICK 1).
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| Isotype |
IgG
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
PICK1
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| Purity |
Immunogen affinity purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
WB: Detects an approx. ~52 kDa protein representing PICK 1 from AtT20 cell extract. ICC: Staining of CV1 cells transfected with PICK 1 results in a diffuse staining pattern when expressed alone and coclustered when coexpressed with mGluR7.
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| Publications |
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Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles.
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| Buffer |
PBS, 1 mg/ml BSA
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| Preservative |
0.05% Sodium Azide
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| Concentration |
1 mg/ml
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| Purity |
Immunogen affinity purified
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Alternate Names for PICK1 Antibody
- alpha binding protein
- dJ1039K5
- MGC15204
- PICK
- PRKCA-binding protein
- PRKCABPprotein interacting with PRKCA
- Protein interacting with C kinase 1
- protein interacting with PRKCA 1
- Protein kinase C-alpha-binding protein
Background
PSD-95/DLG/ZO-1 (PDZ) domain-containing proteins play a central role in synaptic membrane protein localization. The protein interacting with protein kinase C (PICK 1) is a synaptic PDZ domain protein that also contains a coiled-coil and acidic domain. Studies indicate that PICK 1 functions as a targeting and transport protein and has been shown to interact with protein kinase C alpha (PKC alpha), AMPA-type glutamate receptors, and several other membrane receptors via its PDZ domain. The interaction of PICK 1 with PKC alpha is highly dependent on the activation of the kinase. It appears that PICK 1 directs the activated form of PKC alpha to membrane anchored GluR2. Once phosphorylated by PKC alpha, GluR2 is released from the synaptic anchor proteins. Once released, GluR2 is transported from the synaptic membrane in a PICK 1-dependent manner.