PDK-1 Antibody (2D6) Summary
| Immunogen |
Recombinant human PDK1 (29-436aa) purified from E. coli
|
| Specificity |
This antibody is specific for human PDK1. It is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PDK1 protein.
|
| Isotype |
IgG1 Kappa
|
| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Gene |
PDK1
|
| Purity |
Protein G purified
|
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|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:250-1:1000.
|
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
|
| Buffer |
PBS (pH 7.4) and 10% Glycerol
|
| Preservative |
0.02% Sodium Azide
|
| Concentration |
1.0 mg/ml
|
| Purity |
Protein G purified
|
Alternate Names for PDK-1 Antibody (2D6)
- [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial
- EC 2.7.11
- EC 2.7.11.2
- mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1
- PDK1
- PDK-1
- PDPK1
- PKB kinase
- Pyruvate dehydrogenase kinase isoform 1
- pyruvate dehydrogenase kinase, isoenzyme 1
- pyruvate dehydrogenase kinase, isozyme 1
Background
PDK1 (pyruvate dehydrogenase kinase isoform 1) is involved in the regulation of enzymatic activity of mammalian pyruvate dehydrogenase (PDH) that is a part of a mitochondrial multienzyme complex to catalyze the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. PDK1 has been found to serve as an effective therapeutic target for inhibition of glioblastoma growth.