PCNA Antibody Summary
| Immunogen |
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human PCNA protein.
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| Marker |
Proliferation Marker
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| Specificity |
A BLAST analysis was used to suggest cross reactivity with PCNA protein from human, monkey, dog, mouse, rat, bovine, chicken, fish and Xenopus based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
PCNA
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| Purity |
Immunogen affinity purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
This affinity purified antibody has been tested for use in ELISA and western blot.Expect a band approximately 29-36 kDa in size corresponding – PCNA protein by western blotting in the appropriate cell lysate or extract.
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Reactivity Notes
Reactive in human, monkey, dog, mouse, rat, bovine, chicken, fish, Xenopus
Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles.
|
| Buffer |
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
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| Preservative |
0.01% Sodium Azide
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| Purity |
Immunogen affinity purified
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Alternate Names for PCNA Antibody
- cyclin
- DNA polymerase delta auxiliary protein
- MGC8367
- proliferating cell nuclear antigen
Background
The proliferating cell nuclear antigen (PCNA) is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the processibility of DNA polymerase during elongation of the leading strand. PCNA is expressed in the nucleus of all proliferating cells and is pivotal for DNA synthesis and cell cycle progression. In response to DNA damage, PCNA is mono-ubiquitinated and is involved in mismatch-provoked excision. PCNA is a useful marker for DNA synthesis and is highly conserved among most species.