OCT4 Antibody Summary
| Immunogen |
Peptide with sequence C-VTTLGSPMHSN corresponding to C-Terminus according to NP_976034.3.
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| Marker |
Embryonic Stem Cell Marker
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| Specificity |
This antibody is expected to recognise both reported isoforms (NP_002692.2 and NP_976034.3)
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| Clonality |
Polyclonal
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| Host |
Goat
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| Gene |
POU5F1
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| Purity |
Immunogen affinity purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
WB: Approx. 30 kDa band observed in human liver and placenta lysates (calculated MW of 30.1 kDa band according to NP_976034.3).
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| Positive Control |
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| Publications |
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Reactivity Notes
Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine.
Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles.
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| Buffer |
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
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| Preservative |
0.02% Sodium Azide
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| Concentration |
0.5 mg/ml
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| Purity |
Immunogen affinity purified
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Alternate Names for OCT4 Antibody
- class 5, transcription factor 1
- MGC22487
- Oct-3
- OCT3Oct4
- Oct-4
- Octamer-binding protein 3
- otc-4
- OTF3POU domain class 5, transcription factor 1
- OTF4
- POU class 5 homeobox 1
- POU-type homeodomain-containing DNA-binding protein
Background
Stem cells are crucial ingredients in the progressive restriction of cellular developmental capacity during mammalian embryogenesis. Until recently, only embryonic stem cells (ESC) had been shown to exhibit the potential to differentiate into cells of the all three germ layers. However, adult stem cells (ASC) can regain pluripotency (potential to differentiate into various lineages) if transferred from one stem-cell microenvironment into another. The transcription factor OCT4, expressed in ESCs and germ cells, is strongly implicated in the process of maintaining as well as regaining stem-cell pluripotency and functions as a key regulator of mammalian germline development.