Nuf2 Antibody

Product: LY341495

Nuf2 Antibody Summary

Immunogen
The exact sequence is proprietary.
Localization
Nucleus, Kinetochore
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NUF2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.35 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Nuf2 Antibody

  • cancer/testis antigen 106
  • CDCA1hsNuf2
  • cell division cycle associated 1
  • Cell division cycle-associated protein 1
  • CT106
  • hNuf2
  • kinetochore protein Nuf2
  • NUF2, NDC80 kinetochore complex component, homolog (S. cerevisiae)
  • NUF2RhNuf2R

Background

This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Alternatively spliced transcript variants that encode the same protein have been described. [provided by RefSeq]

PMID: 25254944

Nuf2 Antibody

Product: Dinotefuran

Nuf2 Antibody Summary

Immunogen
The immunogen this antibody was made to, maps to a region between residue 414 to 464 of human Nuclear Fallout 2 using the numbering given in entry NP_663735.2 (GeneID 83540).
Predicted Species
Monkey (100%), Primate (100%). Backed by our 100% Guarantee.
Clonality
Polyclonal
Host
Rabbit
Gene
NUF2
Purity
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

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Applications/Dilutions

Dilutions
  • Western Blot 1:1000 – 1:5000
  • Immunoprecipitation 2 – 10 ug/mg lysate

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with Pig, Orangutan, Rhesus Monkey, Gorilla, Chimpanzee, White-tufted-ear marmoset, Crab-eating macaque and Northern white cheeked gibbon.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 – 8.0)
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Nuf2 Antibody

  • cancer/testis antigen 106
  • CDCA1hsNuf2
  • cell division cycle associated 1
  • Cell division cycle-associated protein 1
  • CT106
  • hNuf2
  • kinetochore protein Nuf2
  • NUF2, NDC80 kinetochore complex component, homolog (S. cerevisiae)
  • NUF2RhNuf2R

PMID: 10956229