Nitrotyrosine Antibody (HM11) Summary
| Immunogen |
Nitrated KLH
|
| Specificity |
This highly specific monoclonal antibody HM.11 reacts with nitrotyrosine, both with the free amino acid as well as with proteins containing nitrotyrosine. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic plaques. Nitrotyrosine is formed in tissues in presence of the active metabolite NO and is a stable end product of nitrosylation of tyrosine. Various pathways including the formation of peroxinitrite lead to nitrotyrosine production.
|
| Isotype |
IgG2b
|
| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Purity |
Protein A or G purified
|
| Innovators Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovators Reward
|
Applications/Dilutions
| Dilutions |
|
|
| Application Notes |
Nitrotyrosine is a stable end product of nitrosylation of tyrosine and can be detected by histology on paraffin sections and frozen sections and by Western blot. The antibody is suitable for ELISA applications. (Warning: keep sterile, do not add sodium azide or any nitric containing preservative since this will affect the antibody)
|
|
| Publications |
|
Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles.
|
| Buffer |
0.2 um filtered solution in PBS and 0.1% BSA
|
| Preservative |
No Preservative
|
| Concentration |
0.1 mg/ml
|
| Purity |
Protein A or G purified
|
Alternate Names for Nitrotyrosine Antibody (HM11)
- Nitrotyrosine
Background
The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic placques. Nitrotyrosine is formed in tissues in presence of the active metabolite NO. Various pathways including the formation of peroxinitrite lead to nitrotyrosine production.