Nitrotyrosine Antibody (EM-30)

Product: YH240-EE

Nitrotyrosine Antibody (EM-30) Summary

Immunogen
NO2-Tyr-CH2-Thyroglobulin
Specificity
The antibody EM-30 detects nitrotyrosine group in biological material. It can be used as a marker for peroxynitrite formation in particular tissues
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
Application Notes
This antibody I useful for Western Blot (Positive control:NO2-BSA, NO2-HS) Immunohistochemistry-paraffin( Positive tissue: colon cancer epithelium, prostate hyperplasia)and Immunohistochemistry-frozen (Positive tissue: colon cancer epithelium, prostate hyperplasia)

Reactivity Notes

This antibody reacts to large number of species.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Nitrotyrosine Antibody (EM-30)

  • Nitrotyrosine

Background

Nitrotyrosine can be detected in proteins from a variety of tissues usually in association with pathological conditions. Reaction of nitric oxide with superoxide produces peroxynitrite, which can undergo heterolytic cleavage into nitronium and hydroxyl ions. Nitration of tyrosine residues by nitronium ion forms nitrotyrosine groups in the respective proteins. Nitrotyrosine is thus a marker for inflammation-associated tissue damage.

PMID: 2017149