Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody

Product: Pleuromutilin

Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody Summary

Immunogen
Peptide with sequence C-HVETRAHAEERLLKK corresponding to internal region according to NP_000735.1.
Epitope
internal region
Specificity
This antibody is expected NOT to cross-react with the similar alpha 2 subunit.
Clonality
Polyclonal
Host
Goat
Gene
CHRNA4
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1 – 3 ug/ml
  • Peptide ELISA Detection limit 1:64000
Application Notes
IP: Not suitable. WB: Approx. 70 kDa band observed in rat brain lysates (calculated MW of 70.0 kDa band according to human NP_000735.1and of 70.2 kDa band according to rat NP_077330.1). An additional band of unknown identity was also consistently observed at 22 kDa. This band was successfully blocked by incubation with the immunizing peptide. An anonymous customer has reproted ~75 kDa band in both human and rat tissues as the glycoprotein.
Positive Control
Nicotinic Acetylcholine R alpha 4/CHRNA4 Lysate (NBL1-09181)
Publications
Read Publication using NBP1-00143.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody

  • nAchRa4
  • BFNC
  • cholinergic receptor, nicotinic, alpha 4
  • cholinergic receptor, nicotinic, alpha polypeptide 4
  • CHRNA4
  • EBN
  • EBN1
  • ENFL1
  • FLJ95812
  • nAChR alpha 4
  • NACHR
  • NACHRA4
  • NACRA4
  • neuronal acetylcholine receptor subunit alpha-4
  • neuronal nicotinic acetylcholine receptor alpha-4 subunit
  • Nicotinic Acetylcholine R alpha 4
  • Nicotinic Acetylcholine Ra4

Background

This gene encodes a nicotinic acetylcholine receptor, which belongs to a superfamily of ligand-gated ion channels that play a role in fast signal transmission at synapses. These pentameric receptors can bind acetylcholine, which causes an extensive change in conformation that leads to the opening of an ion-conducting channel across the plasma membrane. This protein is an integral membrane receptor subunit that can interact with either nAChR beta-2 or nAChR beta-4 to form a functional receptor. Mutations in this gene cause nocturnal frontal lobe epilepsy type 1. Polymorphisms in this gene that provide protection against nicotine addiction have been described. [provided by RefSeq]

PMID: 9593974