Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody Summary
| Immunogen |
Peptide with sequence C-HVETRAHAEERLLKK corresponding to internal region according to NP_000735.1.
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| Epitope |
internal region
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| Specificity |
This antibody is expected NOT to cross-react with the similar alpha 2 subunit.
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| Clonality |
Polyclonal
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| Host |
Goat
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| Gene |
CHRNA4
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| Purity |
Immunogen affinity purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
IP: Not suitable. WB: Approx. 70 kDa band observed in rat brain lysates (calculated MW of 70.0 kDa band according to human NP_000735.1and of 70.2 kDa band according to rat NP_077330.1). An additional band of unknown identity was also consistently observed at 22 kDa. This band was successfully blocked by incubation with the immunizing peptide. An anonymous customer has reproted ~75 kDa band in both human and rat tissues as the glycoprotein.
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| Positive Control |
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| Publications |
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Reactivity Notes
Predicted cross-reactivity based on sequence identity: Mouse, Canine, Bovine.
Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles.
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| Buffer |
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
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| Preservative |
0.02% Sodium Azide
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| Concentration |
0.5 mg/ml
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| Purity |
Immunogen affinity purified
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Alternate Names for Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody
- nAchRa4
- BFNC
- cholinergic receptor, nicotinic, alpha 4
- cholinergic receptor, nicotinic, alpha polypeptide 4
- CHRNA4
- EBN
- EBN1
- ENFL1
- FLJ95812
- nAChR alpha 4
- NACHR
- NACHRA4
- NACRA4
- neuronal acetylcholine receptor subunit alpha-4
- neuronal nicotinic acetylcholine receptor alpha-4 subunit
- Nicotinic Acetylcholine R alpha 4
- Nicotinic Acetylcholine Ra4
Background
This gene encodes a nicotinic acetylcholine receptor, which belongs to a superfamily of ligand-gated ion channels that play a role in fast signal transmission at synapses. These pentameric receptors can bind acetylcholine, which causes an extensive change in conformation that leads to the opening of an ion-conducting channel across the plasma membrane. This protein is an integral membrane receptor subunit that can interact with either nAChR beta-2 or nAChR beta-4 to form a functional receptor. Mutations in this gene cause nocturnal frontal lobe epilepsy type 1. Polymorphisms in this gene that provide protection against nicotine addiction have been described. [provided by RefSeq]