NSD3 Antibody [DyLight 405] Summary
| Immunogen |
Synthetic peptide made to an internal portion of the human NSD3 protein (within residues 300-400). [Swiss-Prot# Q9BZ95]
|
| Localization |
Nucleus
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| Specificity |
This has been observed to recognize a band that corresponds to isoform 1 of NSD3.
|
| Predicted Species |
Mouse (100%), Bovine (100%). Backed by our 100% Guarantee.
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
WHSC1L1
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| Purity |
Immunogen affinity purified
|
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Applications/Dilutions
| Dilutions |
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| Application Notes |
This NSD3 antibody is useful for Western blot, where a band is seen at ~165 kDa corresponding to isoform 1 of NSD3.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Theoretical MW |
165 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Reactivity Notes
Predicted to react with mouse and bovine based on 100% sequence homology.
Packaging, Storage & Formulations
| Storage |
Store at 4C in the dark.
|
| Buffer |
50mM Sodium Borate
|
| Preservative |
0.05% Sodium Azide
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| Purity |
Immunogen affinity purified
|
Alternate Names for NSD3 Antibody [DyLight 405]
- EC 2.1.1.43
- FLJ20353
- histone-lysine N-methyltransferase NSD3
- MGC126766
- MGC142029
- NSD3DKFZp667H044
- Nuclear SET domain-containing protein 3
- pp14328
- Protein whistle
- WHSC1-like 1 isoform 9 with methyltransferase activity to lysine
- WHSC1-like protein 1
- Wolf-Hirschhorn syndrome candidate 1-like 1
- Wolf-Hirschhorn syndrome candidate 1-like protein 1
Background
NSD family of SET domain-containing histone methyltransferases includes NSD1, NSD2 (also known as WHSC1, Wolf-Hirschhorn syndrome candidate 1 or MMSET, multiple myeloma SET), and NSD3 (also known as WHSC1L1, WHSC1 like 1). All the three NSD proteins have been directly linked to multiple human diseases and each NSD family member behaves as an oncogene. HSD3 is a istone methyltransferase that preferentially methylates Lys-4 and Lys-27 of histone H3. H3 Lys-4 methylation represents a specific tag for epigenetic transcriptional activation, while Lys-27 is a mark for transcriptional repression. Translocations resulting in NUP98 fusion to NSD1 and NSD3 leads to the development of acute myeloid leukemia (AML) and overexpression of NSD3 via gene amplification occurs in several breast cancer cell lines as well as in non small cell lung carcinomas (NSCLC).