NSD3 Antibody

Product: Columbin

NSD3 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human NSD3. The exact sequence is proprietary.
Localization
Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
WHSC1L1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
162 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Rat (85%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NSD3 Antibody

  • EC 2.1.1.43
  • FLJ20353
  • histone-lysine N-methyltransferase NSD3
  • MGC126766
  • MGC142029
  • NSD3DKFZp667H044
  • Nuclear SET domain-containing protein 3
  • pp14328
  • Protein whistle
  • WHSC1-like 1 isoform 9 with methyltransferase activity to lysine
  • WHSC1-like protein 1
  • Wolf-Hirschhorn syndrome candidate 1-like 1
  • Wolf-Hirschhorn syndrome candidate 1-like protein 1

Background

This gene is related to the Wolf-Hirschhorn syndrome candidate-1 gene and encodes a protein with PWWP (proline-tryptophan-tryptophan-proline) domains. The function of the protein has not been determined. Two alternatively spliced variants have been described.

PMID: 19056862

NSD3 Antibody

Product: Cerdulatinib

NSD3 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:NTQPEQTNAGEVASSLSSTEIRRHSQRRHTSAEEEEPPPVKIAWKTAAARKSLPASITMHKGSLDLQKCNMSPVVKIEQVFALQNATGDGKFIDQFVYSTKGIGNKTEISVRGQDRLIISTPNQRNEKPTQSVSSPEATS
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Predicted Species
Mouse (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
WHSC1L1
Purity
Immunogen affinity purified
Innovators Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1 – 4 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200 – 1:500
Application Notes
For HIER pH6 retrieval is recommended.
Control Peptide
NSD3 Protein (NBP1-89851PEP)

Reactivity Notes

Expected species cross reactivity based on sequence homology: Rat (87%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for NSD3 Antibody

  • EC 2.1.1.43
  • FLJ20353
  • histone-lysine N-methyltransferase NSD3
  • MGC126766
  • MGC142029
  • NSD3DKFZp667H044
  • Nuclear SET domain-containing protein 3
  • pp14328
  • Protein whistle
  • WHSC1-like 1 isoform 9 with methyltransferase activity to lysine
  • WHSC1-like protein 1
  • Wolf-Hirschhorn syndrome candidate 1-like 1
  • Wolf-Hirschhorn syndrome candidate 1-like protein 1

PMID: 9389389

NSD3 Antibody

Product: Sitravatinib

NSD3 Antibody Summary

Immunogen
Synthetic peptide made to an internal portion of the human NSD3 protein (within residues 300-400). [Swiss-Prot# Q9BZ95]
Localization
Nucleus
Specificity
This has been observed to recognize a band that corresponds to isoform 1 of NSD3.
Predicted Species
Mouse (100%), Bovine (100%). Backed by our 100% Guarantee.
Clonality
Polyclonal
Host
Rabbit
Gene
WHSC1L1
Purity
Immunogen affinity purified
Innovators Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Learn about the Innovators Reward

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
Application Notes
This NSD3 antibody is useful for Western blot, where a band is seen at ~165 kDa corresponding to isoform 1 of NSD3. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
165 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
NSD3 Lysate (NBL1-17849)
HeLa Lysate (NB800-PC9)

Reactivity Notes

Predicted to react with mouse and bovine based on 100% sequence homology.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 – 8.0)
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NSD3 Antibody

  • EC 2.1.1.43
  • FLJ20353
  • histone-lysine N-methyltransferase NSD3
  • MGC126766
  • MGC142029
  • NSD3DKFZp667H044
  • Nuclear SET domain-containing protein 3
  • pp14328
  • Protein whistle
  • WHSC1-like 1 isoform 9 with methyltransferase activity to lysine
  • WHSC1-like protein 1
  • Wolf-Hirschhorn syndrome candidate 1-like 1
  • Wolf-Hirschhorn syndrome candidate 1-like protein 1

Background

NSD family of SET domain-containing histone methyltransferases includes NSD1, NSD2 (also known as WHSC1, Wolf-Hirschhorn syndrome candidate 1 or MMSET, multiple myeloma SET), and NSD3 (also known as WHSC1L1, WHSC1 like 1). All the three NSD proteins have been directly linked to multiple human diseases and each NSD family member behaves as an oncogene. HSD3 is a istone methyltransferase that preferentially methylates Lys-4 and Lys-27 of histone H3. H3 Lys-4 methylation represents a specific tag for epigenetic transcriptional activation, while Lys-27 is a mark for transcriptional repression. Translocations resulting in NUP98 fusion to NSD1 and NSD3 leads to the development of acute myeloid leukemia (AML) and overexpression of NSD3 via gene amplification occurs in several breast cancer cell lines as well as in non small cell lung carcinomas (NSCLC).

PMID: 12721103