NOXO1 Antibody Summary
| Immunogen |
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 238-252 of human NOXO1 protein.
|
| Specificity |
A BLAST analysis was used to suggest cross reactivity with NOXO1 protein from human and chimpanzee (100% homology). Expect reactivity with alpha, delta and gamma isoforms of NOXO1. Also expect partial reactivity against NOXO1 homologues from dog and guinea pig (87%), as well as rat (75%) and mouse (68%). Reactivity against homologues from other sources is not known.
|
| Clonality |
Polyclonal
|
| Host |
Rabbit
|
| Gene |
NOXO1
|
| Purity |
Immunogen affinity purified
|
| Innovators Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovators Reward
|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Expect a band approximately 50 kDa in size corresponding – NOXO1 protein by western blotting in the appropriate cell lysate or extract.
|
Reactivity Notes
Reactive in human, chimpanzee
Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles.
|
| Buffer |
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
|
| Preservative |
0.01% Sodium Azide
|
| Purity |
Immunogen affinity purified
|
Alternate Names for NOXO1 Antibody
- MGC20258
- NADPH oxidase organizer 1
- Nox organizer 1
- Nox-organizing protein 1
- P41NOX
- P41NOXA
- P41NOXB
- P41NOXC
- regulatory protein P41NOX
- SH3 and PX domain-containing protein 5
- SH3PXD5NADPH oxidase regulatory protein
- SNX28
Background
NOXO1 (Nox organizing protein 1) and NOXA1 (Nox Activating protein 1) are homologs of p47phox and p67phox. p47phox functions in phagocytes as an essential organizing protein mediating the binding of other regulatory proteins during activation of the phagocyte oxidase, and its translocation to the membrane is triggered upon cell activation by hyperphosphorylation, which relieves autoinhibition of SH3 and PX domains. NOXO1 lacks an auto inhibitory region and phosphorylation sites that are present in p47phox. Co-transfection of Nox1, NOXO1 and NOXA1 reconstitutes ROS (reactive oxygen species) generation in HEK 293 cells in the absence of cell stimulation. NOXO1 binds to the phosphatidylinositol (PtdIns) lipids PtdIns 3,5-P2, PtdIns 5-P and PtdIns 4-P. Unlike p47phox, which is located in the cytosol of resting cells and translocates to the plasma membrane where gp91phox is located, NOXO1 co-localizes with Nox1 in the membranes of resting cells. This localization of NOXO1 is dictated by its PX domain, since this domain but not the remainder of the molecule localizes to membranes.