NKCC1/SLC12A2 Antibody

Product: Glucose-conjugated MGMT inhibitor

NKCC1/SLC12A2 Antibody Summary

Immunogen
Peptide with sequence C-NALQHSIRLSGVEDH corresponding to internal region according to NP_001037.1.
Clonality
Polyclonal
Host
Goat
Gene
SLC12A2
Purity
Immunogen affinity purified
Innovators Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Learn about the Innovators Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:100 – 1:2000
  • Immunohistochemistry 4 – 6 ug/ml
  • Immunohistochemistry-Paraffin 4 – 6 ug/ml
  • Peptide ELISA Detection limit 1:64000
Application Notes
WB: Preliminary experiments gave an approx. 75 kDa band in human, mouse and rat skeletal muscle, heart and brain lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 131 kDa band according to NP_001037.1. The 75 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human colon shows membranous staining.
Publications
Read Publication using NBP1-52148.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for NKCC1/SLC12A2 Antibody

  • Basolateral Na-K-Cl symporter
  • BSC
  • BSC2
  • Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1
  • NKCC1
  • NKCC1MGC104233
  • SLC12A2
  • solute carrier family 12 (sodium/potassium/chloride transporters), member 2
  • solute carrier family 12 member 2

Background

NKCC1 (Na

PMID: 25471355

NKCC1/SLC12A2 Antibody

Product: Rasagiline 13C5 (mesylate racemic)

NKCC1/SLC12A2 Antibody Summary

Immunogen
The immunogen for this antibody was a sequence from 800a.a. to 1000a.a. from NKCC1(a 1212a.a. long protein)
Localization
Membrane; multi-pass membrane protein.
Specificity
Chicken Polyclonal to NKCC1
Clonality
Polyclonal
Host
Chicken
Gene
SLC12A2
Purity
Immunogen affinity purified
Innovators Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Learn about the Innovators Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
Application Notes
Western Blot: E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:2000. Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development.

Reactivity Notes

This antibody is reactive against Human, dog, rat, cow, mouse, chimpanzee.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NKCC1/SLC12A2 Antibody

  • Basolateral Na-K-Cl symporter
  • BSC
  • BSC2
  • Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1
  • NKCC1
  • NKCC1MGC104233
  • SLC12A2
  • solute carrier family 12 (sodium/potassium/chloride transporters), member 2
  • solute carrier family 12 member 2

Background

NKCC1 (Na

PMID: 23505010