NGF R/TNFRSF16/p75NTR Antibody (SPM299) – IHC-Prediluted Summary
| Immunogen |
NGFR from A875 melanoma cells
|
| Localization |
Cell surface and cytoplasmic
|
| Marker |
Soft Tissue Tumor Marker
|
| Specificity |
It recognizes a glycoprotein of 75kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). Its epitope spans in aa 1-160 of extracellular domain of NGFR/NTR. NGF-receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. NGF is important for the development, differentiation, and survival of variety of neuronal and non-neuronal cells. Its action is mediated by binding two distinct receptors, the high affinity p140 and low affinity p75.
|
| Isotype |
IgG1 Kappa
|
| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Gene |
NGFR
|
| Purity |
Protein A or G purified
|
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|
Applications/Dilutions
| Dilutions |
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| Application Notes |
Hu-chromosome location: 17q21-22 Immunohistochemistry-Paraffin 0.5 – 1 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 – 20 min followed by cooling at RT for 20 minutes. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
|
| Theoretical MW |
75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at 4C.
|
| Buffer |
10mM PBS and 0.05% BSA
|
| Preservative |
0.05% Sodium Azide
|
| Purity |
Protein A or G purified
|
Alternate Names for NGF R/TNFRSF16/p75NTR Antibody (SPM299) – IHC-Prediluted
- CD271 antigen
- CD271
- Gp80-LNGFR
- member 16
- nerve growth factor receptor
- NGF R
- NGF receptor
- NGFR
- p75 ICD
- p75 NTR
- TNFRSF16
- TNFRSF16nerve growth factor receptor (TNFR superfamily, member 16)
- tumor necrosis factor receptor superfamily member 16