NFYB Antibody

Product: SYP-5

NFYB Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human NFYB. The exact sequence is proprietary.
Localization
Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NFYB
Purity
Immunogen affinity purified
Innovators Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Learn about the Innovators Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Chromatin Immunoprecipitation
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Rhesus Monkey (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NFYB Antibody

  • CAAT box DNA-binding protein subunit B
  • CBF-A
  • HAP3CBF-B
  • NF-YBCCAAT-binding transcription factor subunit A
  • Nuclear transcription factor Y subunit B
  • nuclear transcription factor Y subunit beta
  • nuclear transcription factor Y, beta
  • Transcription factor NF-Y, B subunit

Background

The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. [provided by RefSeq]

PMID: 10516655

NFYB Antibody

Product: GSK2190916 (sodium salt)

NFYB Antibody Summary

Immunogen
NF-Y (B subunit specific) peptide corresponding to a region near the N-terminal of the human protein, conjugated with KLH.
Localization
Nucleus
Specificity
NF-Y (B subunit specific) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Clonality
Polyclonal
Host
Rabbit
Gene
NFYB
Purity
Delipidation and Defibrination
Innovators Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Learn about the Innovators Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Gel Super Shift Assays 1:1-1:100
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:500
  • Immunoprecipitation 1:10-1:500
Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
No buffer
Preservative
0.01% Sodium Azide
Concentration
90 mg/ml
Purity
Delipidation and Defibrination

Alternate Names for NFYB Antibody

  • CAAT box DNA-binding protein subunit B
  • CBF-A
  • HAP3CBF-B
  • NF-YBCCAAT-binding transcription factor subunit A
  • Nuclear transcription factor Y subunit B
  • nuclear transcription factor Y subunit beta
  • nuclear transcription factor Y, beta
  • Transcription factor NF-Y, B subunit

PMID: 15735050