MyoD Antibody (MYD712) [HRP] Summary
| Immunogen |
Recombinant human MyoD1 protein
|
| Localization |
Nuclear. Only nuclear staining should be considered as evidence of skeletal muscle differentiation.
|
| Marker |
Rhabdomyosarcoma Marker
|
| Specificity |
Recognizes a phosphor-protein of 45kDa, identified as MyoD1. This MAb does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm s tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing s sarcomas and alveolar soft part sarcomas.
|
| Isotype |
IgG1 Kappa
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| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Gene |
MYOD1
|
| Purity |
Protein A purified
|
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|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Immunohistochemistry (Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 1mM EDTA Buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes)
|
| Theoretical MW |
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Readout System |
|
Packaging, Storage & Formulations
| Storage |
Store at 4C in the dark.
|
| Buffer |
PBS
|
| Preservative |
No Preservative
|
| Purity |
Protein A purified
|
Alternate Names for MyoD Antibody (MYD712) [HRP]
- bHLHc1
- bHLHc1BHLHC1
- Class C basic helix-loop-helix protein 1
- MYF3
- MYF3Myf-3
- MyoD
- MYOD1
- MYODmyoblast determination protein 1
- myogenic differentiation 1
- Myogenic factor 3myf-3
- PUM