Mucin 5AC Antibody (1-13M1)

Product: Poloxime

Mucin 5AC Antibody (1-13M1) Summary

Immunogen
M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient.
Localization
Cytoplasmic
Specificity
This MAb recognizes the peptide core of gastric mucin M1 (recently identified as Mucin 5AC).Its epitope is located in the peptide core of MUC5AC. Its epitope is destroyed by beta-mercaptoethanol but not by periodate treatment. MAb 1-13M1 pairs with MAb 9-13M1 to measure MUC5AC protein by ELISA. This mucin is present in primary ovarian mucinous cancer but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Together with a panel of antibodies, Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification of intestinal metaplasia as well as in the identification of pancreatic carcinoma and pre-cancerous changes vs. normal pancreas.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MUC5AC
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5 – 1 ug/million cells in 0.1 ml
  • Immunohistochemistry-Frozen 0.5 – 1.0 ug/ml
  • Immunofluorescence 1 – 2 ug/ml
Application Notes
Hu-chromosome location: 11p15.5
Molecular weight of antigen: >1,000kDa
Immunohistochemistry-Frozen 0.5 – 1 ug/ml for 30 minutes at RT

Reactivity Notes

Human, Monkey, Rabbit, Cat, Mouse, Rat, Cow, Chicken. Does not react with pig, hedgehog. Others-not known.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10mM PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for Mucin 5AC Antibody (1-13M1)

  • gastric mucin
  • leB
  • lewis B blood group antigen
  • major airway glycoprotein
  • MUC5
  • mucin 5, subtypes A and C, tracheobronchial/gastric
  • mucin 5AC, oligomeric mucus/gel-forming pseudogene
  • mucin 5AC, oligomeric mucus/gel-forming
  • mucin-5 subtype AC, tracheobronchial
  • mucin-5AC
  • TBM
  • tracheobronchial mucin

PMID: 9226999