Mouse anti-Human IgG (H+L) Secondary Antibody [Fluorescein]

Product: B-Raf inhibitor

Mouse anti-Human IgG (H L) Secondary Antibody [Fluorescein] Summary

Immunogen
Human IgG whole molecule
Specificity
This product was prepared from monospecific polyclonal ascites by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, Human IgG and Human Serum.
Isotype
IgG
Clonality
Polyclonal
Host
Mouse
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:1:10 – 1:1000
  • Fluorophore-linked immunosorbent assay 1:10000 – 1:50000
  • Immunocytochemistry/Immunofluorescence 1:1000 – 1:5000

Reactivity Notes

Reacts with Human.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA – Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.

Notes

Emission Wavelength: 528, Excitation Wavelength: 495, Label Molecular Weight: 390, Restore with 1.0 mL of deionized water (or equivalent).

PMID: 21602449