Mouse Naive CD8 T Cell Isolation Kit Summary
| Description |
The MagCellect Mouse Naive CD8+ T Cell Isolation Kit is designed to isolate naive CD8+ T cells via a negative selection principle. The resulting cell preparation is highly enriched with naive CD8+ T cells (CD3+/CD8+/CD62L+/CD44low). Typical recoveries range from 5-35% and the purity of recovered cells ranges from 80-90%.
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| Kit Type |
Isolation Kit
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| Gene |
CD8A
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Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze.
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Kit Components
| Components |
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Notes
Other Supplies Required: MagCellect Magnet, Mouse Erythrocyte Lysing Kit, 12 x 75 mm (5 ml) or 17 x 100 mm (15 ml) polystyrene round bottom tubes, Sterile Pasteur pipettes or transfer pipettes or equivalent, Sterile deionized or distilled water, Hanks BSS or equivalent, Bovine serum
Background
CD8 is a heterodimeric glycoprotein consisting of an alpha and beta chain. It is expressed on cytolytic T cells and functions in conjunction with the T cell receptor in the recognition of MHC/peptide complexes. MagCellect products are designed for the isolation of cells in a “liquid phase”. R&D Systems MagCellect technology is based on the use of ferrofluids or magnetic nanoparticles that have no magnetic memory (superparamagnetic), and behave like colloidal particles. This feature allows the ferrofluids to remain in solution without the need for mixing and additionally allows for efficient diffusion kinetics during the binding reaction. The proprietary manufacturing technology of MagCellect Ferrofluids generates particles with higher ligand binding capacity per mass compared to many other larger diameter magnetic particles.