Mouse Memory CD4 T Cell Isolation Kit Summary
| Description |
The MagCellect Mouse Memory CD4+ T Cell Isolation Kit is designed to isolate Memory CD4+ T cells by negative selection. The resulting cell preparation is highly enriched with Memory CD4+ T cells (CD3+/CD4+/CD62L- /CD44high). Typical recoveries range from 5-35% and the purity of recovered cells ranges from 85-95%.
|
| Kit Type |
Isolation Kit
|
| Gene |
CD4
|
Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze.
|
Kit Components
| Components |
|
Notes
Precaution: Some components of this kit contain Sodium Azide, which may react with lead and copper plumbing to form explosive metallic azides. Flush with large volumes of water during disposal. Other Supplies Required: MagCellect Magnet, Mouse Erythrocyte Lysing Kit, 12 x 75 mm (5 ml) or 17 x 100 mm (15 ml) polystyrene round bottom tubes, Sterile Pasteur pipettes or transfer pipettes or equivalent, Sterile deionized or distilled water
Background
CD4 is a transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations. CD4 is expressed along with CD8 on double positive T cells during their development in the thymus. Either CD4 or CD8 expression is then lost, giving rise to single positive (SP) CD4+ or CD8+ mature T cells. CD4+ SP cells, also known as T helper cells, further differentiate into multiple subsets of CD4+ cells including Th1, Th2, Th9, Th17,Th22, Tfh, and Treg cells which regulate humoral and cellular immunity. In human, CD4 is additionally expressed on macrophages, neutrophils, monocytes, NK cells, and neurons and glial cells in the brain. CD4 binds directly to MHC class II molecules on antigen presenting cells. This interaction contributes to the formation of the immunological synapse which is focused around the TCR-MHC class II-antigenic peptide interaction. CD4 also functions as a chemotactic receptor for IL-16 and, in human, as a coreceptor for the gp120 surface glycoprotein of HIV-1. MagCellect products are designed for the isolation of cells in a “liquid phase”. MagCellect technology is based on the use of ferrofluids or magnetic nanoparticles that have no magnetic memory (superparamagnetic) and behave like colloidal particles. This feature allows the ferrofluids to remain in solution without the need for mixing and additionally allows for efficient diffusion kinetics during the binding reaction. The proprietary manufacturing technology of MagCellect Ferrofluids generates particles with higher ligand binding capacity per mass compared to many other larger diameter magnetic particles.