Mouse Erythrocyte Lysing Flow Cytometry Kit Summary
| Description |
For the removal of erythrocytes (RBC) from splenocyte preparations. The Mouse Erythrocyte Lysing Kit is designed to gently lyse erythrocytes from murine spleen cell preparations and stabilize the cells with fixative for later analysis by flow cytometry. Cells exposed to the lysing reagent retain their natural light scattering characteristics and fluorescent staining by flow cytometry. This erythrocyte lysing method also has an advantage over other lysing methods in that the viability of unfixed cells is maintained. The fixative and lysing reagents are provided as separate reagents, thus, allowing cells to be used in tissue culture following RBC lysis. Key Benefits:
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| Kit Type |
Flow Cytometry Kit
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Packaging, Storage & Formulations
| Storage |
Store at room temperature.
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Kit Components
| Components |
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Notes
Why Remove Erythrocytes: The lysis of erythrocytes from whole blood is an important initial step in the isolation and analysis of enriched leukocyte preparations. Recovered immune cells can be accurately characterized following red blood cell removal. Lysis of erythrocytes under conditions that do not disrupt lymphocytes or myeloid cells is critical for downstream applications utilizing leukocytes harvested from whole blood.Other Supplies Required: Deionized or distilled water, 15 ml or 50 ml cetrifuge tubes, Hanks BSS Media, Bovine serum