Mouse B Cell Isolation Kit Summary
| Description |
The MagCellect Mouse B Cell Isolation Kit is designed to isolate B cells via a negative selection principle. The resulting cell preparation is highly enriched with B cells. Typical recovery ranges from 30-55% and the purity of recovered B cells ranges from 90-97%.
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| Kit Type |
Isolation Kit
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Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze.
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Kit Components
| Components |
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Notes
Precaution: Some components of this kit contain Sodium Azide, which may react with lead and copper plumbing to form explosive metallic azides. Flush with large volumes of water during disposal. Other Supplies Required: MagCellect Magnet, Mouse Erythrocyte Lysing Kit, 12 x 75 mm (5 ml) or 17 x 100 mm (15 ml) polystyrene round bottom tubes, Sterile Pasteur pipettes or transfer pipettes or equivalent, Sterile deionized or distilled water
Background
MagCellect products are designed for the isolation of cells in a “liquid phase”. MagCellect technology is based on the use of ferrofluids or magnetic nanoparticles that have no magnetic memory (superparamagnetic) and behave like colloidal particles. This feature allows the ferrofluids to remain in solution without the need for mixing and additionally allows for efficient diffusion kinetics during the binding reaction. The proprietary manufacturing technology of MagCellect Ferrofluids generates particles with higher ligand binding capacity per mass compared to many other larger diameter magnetic particles.