Product: Tolazoline (hydrochloride)
MSH6 Antibody (44) Summary
| Immunogen |
Synthetic human MSH6 peptide.
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| Localization |
Nuclear
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| Specificity |
MSH6 is a heterodimer of MSH2 and binds to DNA containing G/T mismatches. The MSH2-MSH6 complex recognizes a single-based mispair insertion/deletion loop. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related to human carcinogenesis. Studies have shown the mutation of MLH-1, MSH2 and MSH6 genes contribute to the development of sporadic colorectal carcinoma. Repair of mismatch DNA is essential to maintain the integrity of genetic information over time. This antibody cross reacts with mouse, rat, and dog.
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| Isotype |
IgG1
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| Clonality |
Monoclonal
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| Host |
Mouse
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| Gene |
MSH6
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| Purity |
Protein G purified
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| Innovators Reward |
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Applications/Dilutions
| Dilutions |
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| Application Notes |
For IHC: Heat inducted epitope retrieval (1 mM EDTA pH 8.0) is recommended.
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| Positive Control |
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Reactivity Notes
Cross reacts with human, mouse, rat and dog. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze.
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| Buffer |
PBS (pH 7.5), BSA and 6M Urea
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| Preservative |
Sodium Azide
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| Purity |
Protein G purified
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Notes
This product has a prediluted size available. If a 6mL or 7mL pre-diluted size is purchased this product can be used Neat (without further dilution). Use recommended dilution for the concentrated vials only.
Alternate Names for MSH6 Antibody (44)
- DNA mismatch repair protein Msh6
- G/T mismatch-binding protein
- GTBPHNPCC5
- GTMBP
- hMSH6
- HSAP
- mutS (E. coli) homolog 6
- mutS homolog 6 (E. coli)
- MutS-alpha 160 kDa subunit
- p160
- sperm-associated protein
Background
Defects in MSH6 are a cause of hereditary non-polyposis colorectal cancer (HNPCC) (Lynch syndrome). HNPCC is an autosomal, dominantly inherited disease associated with marked increase in cancer susceptibility. it is characterized by a familial predisposition to early onset colorectal carcinoma (crc) and extra-colonic cancers of the gastrointestinal, urological and female reproductive tracts. HNPCC is reported to be the most common form of inherited colorectal cancer in the western world. MSH6 is central to mismatch DNA repair.