MRP4/ABCC4 Antibody (M4I-80)

Product: Nifedipine D8

MRP4/ABCC4 Antibody (M4I-80) Summary

Immunogen
The antibody reacts with the MRP4 antigen.
Specificity
The M4I-80 Mab was selected after immunization with a fusion protein containing the E. coli maltose binding protein and a fragment of the human MRP4 protein corresponding to amino acids 372-431. MRP4 transports cyclic nucleotides and anti-retroviral compounds. The M4I-80 Mab also reacts with the mouse orthologue of the transporter molecule (Mrp4).
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
ABCC4
Purity
Tissue culture supernatant
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Applications/Dilutions

Dilutions
  • Western Blot 1:20-1:50
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:20-1:50
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:20
Application Notes
Immunocytochemistry: use 1:20-50 dil. on acetone fixed cytospin preparations. For immunohistochemistry: M4I- 80 (use 1:20) on acetone fixed frozen sections can be followed by incubation with biotin-labeled rabbit anti-rat IgG (1:100) and HRP-labeled

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.7% BSA
Preservative
0.1% Sodium Azide
Purity
Tissue culture supernatant

Alternate Names for MRP4/ABCC4 Antibody (M4I-80)

  • ABCC4
  • ATP-binding cassette sub-family C member 4
  • ATP-binding cassette, sub-family C (CFTR/MRP), member 4
  • EST170205
  • MOATB
  • MOAT-BbA464I2.1 (ATP-binding cassette, sub-family C (CFTR/MRP), member 4)
  • MOATBcanalicular multispecific organic anion transporter (ABC superfamily)
  • MRP4
  • MRP4MRP/cMOAT-related ABC transporter
  • multidrug resistance-associated protein 4
  • multispecific organic anion transporter B
  • Multi-specific organic anion transporter B

Background

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. The specific function of this protein has not yet been determined; however, this protein may play a role in cellular detoxification as a pump for its substrate, organic anions. Alternative splicing results in multiple splice variants encoding different isoforms.

PMID: 19056863