MARCKS Antibody Summary
| Immunogen |
The antiserum was produced against synthesized non-phosphopeptide derived from human MARCKS around aa. 158 (R-F-S-F-K).
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| Specificity |
MARCKS (specific to a.a. 158) detects endogenous levels of total MARCKS protein.
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
MARCKS
|
| Purity |
Immunogen affinity purified
|
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Applications/Dilutions
| Dilutions |
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| Application Notes |
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
|
| Theoretical MW |
80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Reactivity Notes
This antibody is reactive against Human, Mouse, Rat
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
|
| Buffer |
phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, and 50% glycerol.
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| Preservative |
0.02% Sodium Azide
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| Concentration |
1.0 mg/ml
|
| Purity |
Immunogen affinity purified
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Alternate Names for MARCKS Antibody
- 80K-L protein
- 80K-L
- FLJ14368
- MACS
- MACSmyristoylated alanine-rich C-kinase substrate
- MARCKS
- MRACKS
- myristoylated alanine-rich protein kinase C substrate (MARCKS, 80K-L)
- myristoylated alanine-rich protein kinase C substrate
- phosphomyristin
- PKCSLFLJ90045
- PRKCSL
- Protein kinase C substrate, 80 kDa protein, light chain
Background
MARCKS, (Myristoylated Alanine-Rich C Kinase Substrate), is a member of a family of calmodulin binding proteins and is a major substrate for phosphorylation by protein kinase C (PKC). The phosphorylation of Ser152/156 can be used as a measure of PKC activation. Phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin.