Lightning-Link PerCP Antibody Labeling Kit Summary
| Description |
Lightning-Link antibody labeling kits enable the direct labeling of antibodies, proteins, peptides or other biomolecules for use in R&D applications, drug discovery and the development of diagnostic kits.
Our PerCP antibody labeling kit enables the direct conjugation of PerCP to any biomolecule with an available amine group. The researcher simply pipettes the antibody or other biomolecule into the vial of Lightning-Link PerCP and incubates for 3 hours.
PerCP is a small 35kDa phycobiliprotein isolated from red algae. It has a strong absorption peak at 484nm and a large stokes shift (194nm) with its maximum emission peak at 678nm. PerCP is commonly used for fluorescent immunolabeling, particularly in applications involving fluorescent-activated cell sorting. Popular conjugates applications: |
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| Kit Type |
Antibody Labeling Kit
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Applications/Dilutions
| Application Notes |
This kit is useful at directly conjugating primary antibody and other proteins.
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Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles.
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| Buffer |
This kit contains 3x10ug reactions.
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Kit Components
| Components |
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Notes
For more information please check out these useful links!
Fluorescent Labels Poster
Antibody Labeling Guide
Antibody Purification Guide
Lightning Link® is a registered trademark of Innova Biosciences.
Background
The Lightning-Link conjugation system represents a quantum leap forward in conjugation technology. It allows you to make antibody conjugates with minimal hands-on time – less than 30 seconds. Lightning-Link simplifies immunoassay techniques, such as western blotting, ELISA and immunohistochemistry, by eliminating secondary reagents and cutting the number of incubation and wash steps.
Despite its simplicity, Lightning-Link is a very sophisticated conjugation system in which the antibody is directionally coupled to the label (and not to itself) in a controlled fashion, creating high quality conjugates. As the procedure is not interrupted by desalting steps, trial conjugates can be prepared with microgram quantities of protein and then scaled up with ease. Lightning-Link eliminates problems associated with scale up; hands-on time is essentially independent of process scale. Consistency from batch to batch is easy to achieve, and recoveries approach 100%.