Kappa Light Chain Antibody (HP6053) – Azide and BSA Free Summary
Immunogen |
Purified human Ig kappa chain
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Localization |
Cell Surface, cytoplasmic and secreted
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Marker |
B-Cell Marker
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Specificity |
This MAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
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Isotype |
IgG1 Kappa
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Clonality |
Monoclonal
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Host |
Mouse
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Gene |
IGKC
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Purity |
Protein A purified
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Applications/Dilutions
Dilutions |
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Application Notes |
Immunohistochemistry (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Theoretical MW |
22.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Buffer |
10mM PBS – BSA free
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Preservative |
No Preservative
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Concentration |
1.0 mg/ml
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Purity |
Protein A purified
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Alternate Names for Kappa Light Chain Antibody (HP6053) – Azide and BSA Free
- HCAK1
- IGKCD
- immunoglobulin kappa constant
- Km
- MGC111575
- MGC62011
- MGC72072
- MGC88770
- MGC88771
- MGC88809