Product: HLCL-62 (hydrochloride)
Kappa Light Chain Antibody (4G7) Summary
| Immunogen |
Mixture of light chains of human immunoglobulin.
|
| Specificity |
Specific for independent constantly expressed epitope of kappa chain. Reacts with free and heavy chain-associated kappa chains.
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| Isotype |
IgG2a
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| Clonality |
Monoclonal
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| Host |
Mouse
|
| Gene |
IGKC
|
| Purity |
Protein A purified
|
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|
Applications/Dilutions
| Dilutions |
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| Application Notes |
Detection of kappa-chains or kappa-immunoglobulin in ELISA. MAb 4G7 can be used for coating and as conjugate. It also works in both cryostat or paraffin-embedded sections.
|
Reactivity Notes
Cross-reacts with Human.Not yet tested in other species.
Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze.
|
| Buffer |
PBS (pH 7.4)
|
| Preservative |
0.09% Sodium Azide
|
| Purity |
Protein A purified
|
Alternate Names for Kappa Light Chain Antibody (4G7)
- HCAK1
- IGKCD
- immunoglobulin kappa constant
- Km
- MGC111575
- MGC62011
- MGC72072
- MGC88770
- MGC88771
- MGC88809
Background
Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkins lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83.