KMT1A/SUV39H1 Antibody (44.1)

Product: 5-Hydroxy Propafenone (D7 Hydrochloride)

KMT1A/SUV39H1 Antibody (44.1) Summary

Immunogen
Recombinant fusion protein, MBP-SUV39H1.
Localization
Nuclear; associates with centromeric constitutive heterochromatin
Specificity
Monoclonal Anti-SUV39H1 Histone Methyltransferase recognizes an epitope in the N-terminal (195 amino acids) of human and mouse SUV39H1 Histone Methyltransferase.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SUV39H1
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Western Blot 2-4 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:10-1:500
Positive Control
HeLa Lysate (NB800-PC1)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10mM PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
2.0 mg/ml
Purity
Protein A or G purified

Alternate Names for KMT1A/SUV39H1 Antibody (44.1)

  • H3-K9-HMTase 1
  • Histone H3-K9 methyltransferase 1
  • histone-lysine N-methyltransferase SUV39H1
  • histone-lysine N-methyltransferase, H3 lysine-9 specific 1
  • KMT1AEC 2.1.1.43
  • Lysine N-methyltransferase 1A
  • MG44
  • Position-effect variegation 3-9 homolog
  • Su(var)3-9 homolog 1
  • suppressor of variegation 3-9 (Drosophila) homolog 1
  • suppressor of variegation 3-9 homolog 1 (Drosophila)
  • Suppressor of variegation 3-9 homolog 1
  • Suppressor of variegation 3-9 homolog 1, Suvar) 3-9 homolog
  • SUV39H

Background

In eukaryotic cells the histone methylase KMT1A / SUV39H1 and the methyl-lys binding protein HP1 interact to repress transcription at heterochromatic sites. Lys 9 of histone H3 is methylated by KMT1A, creating a binding site for the chromo domain of HP1. The Lys methylase activity of KMT1A resides in the SET domain. KMT1A and HP1 are both involved in the repressive functions of the retinoblastoma (Rb) protein. KMT1A cooperates with Rb to repress the cyclin E promoter, and in fibrobroblasts that are disrupted for KMT1A, the activity of the cyclin E and cyclin A2 genes are specifcally elevated. Chromatin IPs (ChIPs) show that Rb is necessary to direct methylation of histone H3, and is necessary for binding of HP1 to the cyclin E promoter. The KMT1A-HP1 complex is thus not only involved in heterochromatic silencing but also has a role in repression of euchromatic genes by Rb and perhaps other co-repressor proteins. Histone H3 lysine 9 can be mono, di or tri methylated. Different HMTases have recently been found responsible for each modification state. G9a is thought to be responsible for all mono and di methylation of H3 K9 in slient regions of heterochromatin. In contrast, KMT1A and H2 are thought to be responsible fro the tri methylation of H3K9 found in pericentric heterochromatin.

PMID: 19766403