Integrin beta 6 Antibody (CSbeta6)

Product: Vipadenant

Integrin beta 6 Antibody (CSbeta6) Summary

Immunogen
beta 6 knockout mice have been immunized with recombinant secreted human alpaVbeta6.
Specificity
Monoclonal antibody MAB2076Z, raised by immunizing beta6 knockout mice with recombinant secreted human alphavbeta6, is useful for flow cytometry and immunoprecipitation in all species tested, including mouse. It also works well for immunohistochemistry of acetone-fixed tissues in several species, including rat, rabbit, monkey, and human. The antibody does not work well for immunohistochemistry of mouse tissue. This antibody does not recognize the aV subunit and does not react with alphaV integrins other than alphavbeta6. Because the antibody is not effective in western Blot, it is unknown whether the epitope recognized by the antibody localizes to the alphavbeta6 heterodimer or to the beta6 subunit only.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ITGB6
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
Immunohistochemistry (acetone fixation): Effective for Rabbit, rat, monkey, and human. Not effective for mouse tissue. Immunocytochemistry/Immunofluorescence : Effective for F6, HT-29, H441 cell lines (others not tested). Flow cytometry: Effective for all species tested, including rabbit, rat, monkey, human, and mouse. Immunoprecipitation

Reactivity Notes

Human, Monkey, Rat, Rabbit

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A or G purified

Notes

Relevant Entrez Gene Numbers: NM_000888.3

Alternate Names for Integrin beta 6 Antibody (CSbeta6)

  • Integrin beta 6
  • integrin beta-6
  • integrin, beta 6
  • ITGB6

PMID: 12825931