Integrin beta 1/CD29 Antibody (JB1B)

Product: S1P3 Agonist III

Integrin beta 1/CD29 Antibody (JB1B) Summary

Immunogen
Purified Human beta1 integrin
Marker
Stem Cell Marker
Specificity
NB100-64804 reacts with Human Integrin beta1 as confirmed with beta1 transfectants and by reciprocal preclearing of beta1 reactivity with anti-beta1. The antibody stimulates the adherence of several cell types. The removal of azide is recommended prior to use in functional assays.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ITGB1
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 0.1 ug/ml-1 ug/ml
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 5 ug/ml
  • Immunoprecipitation 1-5 ug
Positive Control
Integrin beta 1/CD29 Lysate (NBL1-12069)

Reactivity Notes

Reacts with Human. Cross reacts with Cynomolgus monkey and Rhesus Monkey. Does not react with Rat. Reacts weakly with Pig.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Phosphate buffered saline
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for Integrin beta 1/CD29 Antibody (JB1B)

  • CD29 antigen
  • CD29
  • Fibronectin receptor subunit beta
  • FNRBVLAB
  • GPIIA
  • Integrin beta 1
  • integrin beta-1
  • integrin VLA-4 beta subunit
  • integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12)
  • ITGB1
  • MDF2
  • MSK12
  • very late activation protein, beta polypeptide
  • VLA-4 subunit beta
  • VLA-BETA

Background

Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6, also known as CD49a, CD49b, CD49c, CD49d, CD49e, and CD49f, respectively) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. The clustering of integrins on a cell surface leads to the formation of focal contacts. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression.

PMID: 10027919