Product: Calcitriol Impurities A
Integrin alpha 5/CD49e Antibody (HMa5-1) [FITC] Summary
| Immunogen |
The immunogen for this antibody was CD49e.
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| Isotype |
IgG
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| Clonality |
Monoclonal
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| Host |
Armenian Hamster
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| Gene |
ITGA5
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| Purity |
Protein A or G purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
This HMa5-1 antibody has been tested by flow cytometric analysis of mouse bone marrow cell suspensions. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 24256316)
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Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze.
|
| Buffer |
Aqueous buffer, may contain carrier protein/stabilizer (proprietary)
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| Preservative |
0.09% Sodium Azide
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| Concentration |
0.5 mg/ml
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| Purity |
Protein A or G purified
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Alternate Names for Integrin alpha 5/CD49e Antibody (HMa5-1) [FITC]
- CD49 antigen-like family member E
- CD49e antigen
- CD49e
- Fibronectin receptor subunit alpha
- fibronectin receptor, alpha subunit
- FNRAVLA5A
- Integrin alpha 5
- integrin alpha-5
- Integrin alpha-F
- integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
- ITGA5
- very late activation protein 5, alpha subunit
- VLA-5 alpha
- VLA-5
Background
The HMalpha5-1 monoclonal antibody reacts with mouse and rat CD49e, also known as integrin alpha5 or VLA-5A. CD49e is a member of the integrin alpha chain family, and binds with CD29 (integrin beta1) to form the receptor VLA-5, which binds the ligands fibronectin and fibrinogen. CD49e is a type I, single-pass membrane protein, with a molecular weight of 135 kDa, and is expressed on multiple cell types including thymocytes, mast cells, activated T cells and splenic B cells. In addition to its role in adhesion, VLA-5 contributes to T-cell co-stimulation. The HMalpha5-1 monoclonal antibody has been demonstrated to interfere with VLA-5-mediated cell adhesion, and with the degranulation of IgE-sensitized rat RBL cells stimulated on fibronectin-coated plates. Furthermore, HMalpha5-1 injected into mice subcutaneously, along with anti-CD49d and anti-CD61, is able to inhibit passive cutaneous anaphylaxis.