Integrin alpha 4/CD49d Antibody Summary
| Immunogen |
Antibody was raised against a 16 amino acid synthetic peptide from near the center of human Integrin alpha 4. The immunogen is located within amino acids 150 – 200 of Integrin alpha 4.
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| Specificity |
At least two isoforms of integrin alpha 4 are known to exist.
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
ITGA4
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| Purity |
Immunogen affinity purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
Integrin alpha 4 antibody can be used for the detection of Integrin alpha 4 by Western blot at 0.5 – 1 mg/ml.
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| Theoretical MW |
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Control Peptide |
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Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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| Buffer |
PBS
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| Preservative |
0.02% Sodium Azide
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| Concentration |
1 mg/ml
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| Purity |
Immunogen affinity purified
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Alternate Names for Integrin alpha 4/CD49d Antibody
- 269C wild type
- CD49 antigen-like family member D
- CD49d antigen
- CD49d
- CD49Dantigen CD49D, alpha-4 subunit of VLA-4 receptor
- IA4
- Integrin alpha 4
- integrin alpha-4 subunit
- integrin alpha-4
- Integrin alpha-IV
- integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)
- ITGA4
- MGC90518
- very late activation protein 4 receptor, alpha 4 subunit
- VLA-4 subunit alpha
- VLA-4
Background
The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains. Alpha4 chain associates with either beta1 or beta7 chain. It has been demonstrated that the putative ligand-binding sites of both integrin alpha4beta1 and alpha4beta7 is located on the alpha4 chain. These ligands included Madcam, VCAM, and fibronectin. Madcam is known as the principal ligand for integrin alpha4beta7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin alpha4beta1, the gut mucosal homing receptor for peripheral T cells. Despite its predicted molecular weight, Integrin alpha4 will often run at higher molecular weight in SDS-PAGE. At least two isoforms are known to exist.